A Simple Application Technique for Paper Chromatography
نویسندگان
چکیده
منابع مشابه
A Simple, Rapid Method for Paper Chromatography of Barbiturates.
Barbiturates are extracted quantitatively into ethyl ether from biologic fluids buffered to pH 6. A measured portion of the ether extract is then impinged to dryness and the residue spotted on Whatman No. 2 paper that has been previously dipped in alkali and dried. The paper is developed by descending chromatography with chloroform saturated with water. The barbiturate may then be located and a...
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متن کاملThe application of simple paper chromatography to the study of glutathione in the rat epidermis.
The use of paper chromatography has been demonstrated to be a simple and efficient means for the detection and separation of glutathione from the constituent amino acids and the related metabolite. The use of Nethylmalemide to stabilize the sulfhydryl group during chromatography was found to be a marked advance. This technic was applied to the study of glutathione in the epidermis. The incorpor...
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When volatile solvents are used for paper chromatography, as in the systems described by Bush (1952) for separation of steroids, it is usually necessary to hang the paper in the tank for several hours' equilibration before the addition of the mobile phase. If equilibration is allowed to proceed overnight much of the next day will be taken up with running the chromatogram. An apparatus has there...
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ژورنال
عنوان ژورنال: Nature
سال: 1952
ISSN: 0028-0836,1476-4687
DOI: 10.1038/169672a0